Originally isolated from a metastatic bone tumour biopsy from a 4 year old female child suffering from neuroblastoma a rare nerve cell cancer2. SH-SY5Y is a thrice-subcloned cell line derived from the SK-N-SH neuroblastoma cell line.

Sh Sy5y Wikipedia

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Sh sy5y cell lines. A subline of the neuroblastoma SK-N-SH line derived from a metastatic bone tumor. SH-SY5Y is a thrice cloned SK-N-SH - SH-SY - SH-SY5 - SH-SY5Y subline of the neuroblastoma cell line SK-N-SH see ATCC HTB-11 which was established in 1970 from a metastatic bone tumor. Cell Line Description SH-SY5Y is a thrice-cloned sub-line of bone marrow biopsy-derived line SK-N-SH Sigma catalogue no.

Asked 10th Jun 2013. The SH-SY5Y cell line is a thrice cloned subline of SK-N-SH cells which were originally established from a bone marrow biopsy of a neuroblastoma patient with sympathetic adrenergic ganglial origin in the early 1970s. Cell Lines S sh-sy5y Cells.

First to SH-SY then to SH-SY5 and finally to SH-SY5Y. TOKU-E is a global leader in biotechnology innovation offering great benefits and applications to the biopharmaceutical and diagnostic industries as well as for biotechnology research communities. Originally derived from a metastatic bone tumor biopsy SH-SY5Y ATCC CRL-2266 cells are a subline of the parental line SK-N-SH ATCC HTB-11.

SH-SY5Y is a thrice-cloned sub-line of bone marrow biopsy-derived line SK-N-SH ECACC catalogue no. For the cell viability assays the cells were seeded to 24-well plates 2104cellwell. The human neuroblastoma clonal SH-SY5Y cell line was obtained from American Type Culture Collection Figure 812.

What is the best cell density of SH-SY5Y cell line to perform a MTT assay in 96 well plate. It serves as a model for neurodegenerative disorders since the cells can be converted to various types of functional neurons by the addition of specific compounds. Cell culture protocol for SH-SY5Y neuroblastoma cells human ATCC CRL-2266 Growth medium.

This project is supported byTOKU-Ewhich specializes in manufacturing ultra-pure antibiotics for a broad spectrum of biotechnology applications as well as for the pharmaceutical industry. Is a 11 ratio of ATCC-formulated MEM and F12 Invitrogen MEM 11095-080 with L-Glutamate Additives- 1. However the use of an oncogenically transformed cell line with catecholaminergic rather than exclusively DAergic properties remains a controversial issue in the PD field.

Although these properties do not classify SH-SY5Y cells as purely DAergic this cell line has been widely used as a model for PD. Cultures were maintained in serum-containing medium 11 mixture of Dulbeccos modified Eagles medium and Hams nutrient mixture F-12 supplemented with 15 fetal bovine serum and MEM nonessential amino acids. Neuroblast from neural tissue 94030304.

1x Non Essential amino acids 100x stock 1100dilution- Cell Gro25-025-CI. 1 Product Result Match Criteria. For example these cells express tyrosine hydroxylase and dopamine-beta-hydroxylase as well as the dopamine transporter.

SH-SY5Y Cell Line human. I have been trying to transfect SH-SY5Y cells by using more than one method and the reagent such as TransFast Promega linear PEI polyethylenimine and Amaxa Cell Line Nucleofector Kit V. Grows as a mixture of floating and adherent cells.

SH-SY5Y is a human derived cell line used in scientific research. They are adrenergic in phenotype but also express dopaminergic markers and as such have been used to study Parkinsons disease neurogenesis and other characteristics of brain cell. SH-SY5Y cells were derived through three successive sub-clones of the parental SK-N-SH cell line1.

SH-SY-5Y has dopamine-beta-hydroxylase activity and can convert glutamate to the neurotransmitter GABA. The SH-SY5Y cell line displays a number of genetic aberrations due to its cancerous origin but most genes and pathways dysregulated in PD pathogenesis are intact. The SH-SY5Y cell line has become a popular cell model for PD research because this cell line posses many characteristics of DAergic neurons.

SH-SY5Y cells are often used as in vitro models of neuronal function and differentiation. SH-SY-5Y has dopamine-v-hydroxylase activity and can convert glutamate to the neurotransmitter GABA. I am trying to set up a MTT assay.

Will form tumours in nude mice in approximately 3-4 weeks. SK-N-SH were subcloned three times. 4 This cell line has been widely used as model of neurons since the early 1980s as these cells posses many biochemical and functional properties of neurons.

Human neuroblastoma SH-SY5Y cells ECACC UK were cultured up to 72h in DMEMF12 containing 10 FBS 1 stable glutamine and antibiotics. Product Name Description details. The original cell line called SK-N-SH from which it was subcloned was isolated from a bone marrow biopsy taken from a four-year-old female with neuroblastoma.

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